Multiple forms of endo-polygalacturonase from Saccharomyces fragilis.
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چکیده
منابع مشابه
Comprehensive glycan analysis of recombinant Aspergillus niger endo-polygalacturonase C.
The enzyme PGC is produced by the fungus Aspergillus niger during invasion of plant cell walls. The enzyme has been homologously overexpressed to provide sufficient quantities of purified enzyme for biological studies. We have characterized this enzyme in terms of its posttranslational modifications (PTMs) and found it to be both N- and O-glycosylated. The glycosyl moieties have also been chara...
متن کاملSeparation of endo-polygalacturonase using aqueous two-phase partitioning.
The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)-polyvinyl alcohol (PVA10000) and PEG-hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000-PVA10000 system, endo-PG presented in the ferm...
متن کاملEnzyme Formation in Saccharomyces fragilis
Krebs, H. A. & Johnson, W. A. (1937). Enzymologia, 4, 148. Lardy, H. A. (1949). In Manometric Techniques and Tis8ue Metaboli8m, 2nd ed. p. 209. Ed. by Umbreit, W. W., Burris, R. H. & Stauffer, J. F. Minneapolis: Burgess Publ. Co. Lipmann, F., Kaplan, N. 0., Novelli, G. D. & Tuttle, L. C. (1950). J. biol. Chem. 186, 235. Massey, V. & Rogers, W. P. (1950). Aust. J. 8ci. Res. Ser. B, 3, 251. Natel...
متن کاملLactose Utilization and Hydrolysis in Saccharomyces Fragilis.
SUMMARY Sodium azide, 2,4-dinitrophenol and iodoacetate did not inhibit hydrolysis of lactose by cell-free preparations of Sacchuromyces fragilis ,8-galactosidase, but with intact organisms fermentation and hydrolysis were inhibited to a similar extent. This suggests that these inhibitors may interfere with the transport of lactose into the cell. Galactose fermentation was inhibited by sodium a...
متن کاملRelationships among endo-polygalacturonase, oxalate, pH, and plant polygalacturonase-inhibiting protein (PGIP) in the interaction between Sclerotinia sclerotiorum and soybean.
The necrotrophic fungal pathogen Sclerotinia sclerotiorum secretes oxalic acid and endo-polygalacturonase (endo-PG) in host plants. Oxalic acid acidifies the plant tissue to values more suitable to endo-PG activity. However, we observed that the infected soybean seedlings possessed a pH of 3.8, which is below that optimal for endo-PG activity (4.5 to 5.0). We investigated, therefore, the effect...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1980
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.44.473